Ferrostatin-1 (Fer-1) （CAS#347174-05-4 Cat#D910380） ferroptosis inhibitor
|Appearance||faint purple to brown|
|Solubility||≥ 9.8 mg/mL in DMSO, ≥ 99.6 mg/mL in EtOH with ultrasonic|
|Biological Target||Ferroptosis inhibitor, erastin-induced|
|Storage||4°C, protect from light|
Biological Target ：
Ferroptosis inhibitor, erastin-induced,Ferrostatin-1 is a potent inhibitor of ferroptosis with an EC50 of 60 nM.
In vitro activity ：
Ferrostatin-1 is the most potent inhibitor of erastin-induced ferroptosis in HT-1080 cells (EC50=60 nM). Ferrostatin-1 does not inhibit ERK phosphorylation or arrest the proliferation of HT-1080 cells. Ferrostatin-1 does, however, prevent erastin-induced accumulation of cytosolic and lipid ROS. Cells pretreated with 0.4 μM Ferrostatin-1 displays significantly reduce intracellular reactive oxygen species (ROS) and nitrogen species (RNS) below basal levels. Additionally, increased intracellular ROS levels are also significantly lowered below basal levels by a 0.4 µM Ferrostatin-1 pretreatment. Ferrostatin-1 treatment for 24 h does not change the expression level of i-NOS in SHSY-5Y cell when compare with vehicle (0.02 % DMSO) treated cells.
Kinase Assay: The cells treated for 24 h with Ferrostatin-1 are washed three times with phosphate-buffered saline (PBS) and pelleted by centrifugation. The supernatant is removed and 80 μL of lysis buffer is added to the cells and then stored overnight at -20°C. Subsequently, the cells are centrifuged at 10,000 RPM for 12 min and both the pellet and supernatant are stored for future use.
Cell Assay: SH-S5Y cells are seeded in 24-well plates. After overnight incubation, the cells are exposed for 24 h to 1 µM of Ferrostatin-1, under standard growth conditions. Bright field photomicrographs are captured using an inverted microscope equipped with 10× objective in a live-cell modality.
. Dixon SJ, et al. Ferroptosis: an iron-dependent form of nonapoptotic cell death. Cell. 2012 May 25;149(5):1060-72.
. Kabiraj P, et al. The neuroprotective role of ferrostatin-1 under rotenone-induced oxidative stress in dopaminergic neuroblastoma cells. Protein J. 2015 Oct;34(5):349-58.
Max Conc. mg/mL
Max Conc. mM
DMF:PBS (pH 7.2) (1:4)
Concentration / Solvent Volume / Mass
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