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Home Metabolism Ferroptosis Ferrostatin-1 (Fer-1) (CAS#347174-05-4 Cat#D910380)
Ferroptosis inhibitor, erastin-induced,Ferrostatin-1 is a potent inhibitor of ferroptosis with an EC50 of 60 nM.

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5mg $ 61.00 100
25mg $ 180.00 100
100mg $ 564.00 100
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Overview
Synonym Ferrostatin-1; Frer-1
Appearance faint purple to brown
Purity >99%
CAS# 347174-05-4
Formula C₁₅H₂₂N₂O₂
Molecular Weight 262.35
Solubility ≥ 9.8 mg/mL in DMSO, ≥ 99.6 mg/mL in EtOH with ultrasonic
Biological Target Ferroptosis inhibitor, erastin-induced
Storage 4°C, protect from light
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Details
  • Biological Activity


Biological Target

Ferroptosis inhibitor, erastin-induced,Ferrostatin-1 is a potent inhibitor of ferroptosis with an EC50 of 60 nM.


In vitro activity :


    Ferrostatin-1 is the most potent inhibitor of erastin-induced ferroptosis in HT-1080 cells (EC50=60 nM). Ferrostatin-1 does not inhibit ERK phosphorylation or arrest the proliferation of HT-1080 cells. Ferrostatin-1 does, however, prevent erastin-induced accumulation of cytosolic and lipid ROS[1]. Cells pretreated with 0.4 μM Ferrostatin-1 displays significantly reduce intracellular reactive oxygen species (ROS) and nitrogen species (RNS) below basal levels. Additionally, increased intracellular ROS levels are also significantly lowered below basal levels by a 0.4 µM Ferrostatin-1 pretreatment. Ferrostatin-1 treatment for 24 h does not change the expression level of i-NOS in SHSY-5Y cell when compare with vehicle (0.02 % DMSO) treated cells[2].


Kinase Assay:    The cells treated for 24 h with Ferrostatin-1 are washed three times with phosphate-buffered saline (PBS) and pelleted by centrifugation. The supernatant is removed and 80 μL of lysis buffer is added to the cells and then stored overnight at -20°C. Subsequently, the cells are centrifuged at 10,000 RPM for 12 min and both the pellet and supernatant are stored for future use[2].



Cell Assay:    SH-S5Y cells are seeded in 24-well plates. After overnight incubation, the cells are exposed for 24 h to 1 µM of Ferrostatin-1, under standard growth conditions. Bright field photomicrographs are captured using an inverted microscope equipped with 10× objective in a live-cell modality[2].



  • Validation&References


Validation :

References :

[1]. Dixon SJ, et al. Ferroptosis: an iron-dependent form of nonapoptotic cell death. Cell. 2012 May 25;149(5):1060-72.

[2]. Kabiraj P, et al. The neuroprotective role of ferrostatin-1 under rotenone-induced oxidative stress in dopaminergic neuroblastoma cells. Protein J. 2015 Oct;34(5):349-58.


  • Solubility & Formulation

    Solubility Data

    Solvent

    Max Conc. mg/mL

    Max Conc. mM

    Solubility

    DMSO

    53.31

    203.2

    DMF

    30.0

    114.35

    DMF:PBS (pH 7.2) (1:4)

    0.2

    0.76

    Ethanol

    29.41

    112.1

    Preparing Stock Solutions

    Concentration / Solvent Volume / Mass

    1 mg

    5 mg

    10 mg

    1 mM

    3.81 mL

    19.06 mL

    38.12 mL

    5 mM

    0.76 mL

    3.81 mL

    7.62 mL

    10 mM

    0.38 mL

    1.91 mL

    3.81 mL

    50 mM

    0.08 mL

    0.38 mL

    0.76 mL


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PLEASE KEEP THE PRODUCT UNDER -20°C FOR LONG-TERM STORAGE.


NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.


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We ensure that the product is shipped under conditions that will maintain the quality of the reagents. Upon receipt of the product, follow the storage recommendations on the product datasheet.

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